Knowledge Base

Everything you need to understand plasma technology all in one place.

Knowledge Articles

Investigating the nanoscale adhesion mechanisms between polymeric biomaterials and biological samples

Recently published research from scientists at Queen Mary University of London and GlaxoSmithKline demonstrates the complex nature of nanoscale adhesion mechanisms between polymeric biomaterials and biological samples.  Henniker’s HPT-200 plasma system is used in this work to provide the clean substrate surfaces (silicon and gold) onto which a variety of polymer brushes and self-assembled monolayers (SAMs) were synthesized.

Please find the abstract below;

The physico-chemistry of adhesions of protein resistant and weak polyelectrolyte brushes to cells and tissues

[All information courtesy of https://pubs.rsc.org/  – Edward J. Cozens ab, Dexu Kong ab, Nima Roohpour c, and Julien E. Gautrot | Received 11th July 2019, Accepted 15th October 2019 | First published on 5th December 2019]

Investigating the nanoscale adhesion mechanisms between polymeric biomaterials and biological samples - image of paper

Abstract

The non-specific adhesion of polymers and soft tissues is of great interest to the field of biomedical engineering, as it will shed light on some of the processes that regulate interactions between scaffolds, implants, and nanoparticles with surrounding tissues after implantation or delivery. In order to promote adhesion to soft tissues, a greater understanding of the relationship between polymer chemistry and nanoscale adhesion mechanisms is required.

In this work, we grew poly(dimethylaminoethyl methacrylate) (PDMAEMA), poly(acrylic acid) (PAA) and poly(oligo ethylene glycol methacrylate) (POEGMA) brushes from the surface of silica beads and investigated their adhesion to a variety of substrates via colloidal probe-based atomic force microscopy (AFM). We first characterized adhesion to a range of substrates with defined surface chemistry (self-assembled monolayers (SAMs) with a range of hydrophilicities, charge and hydrogen bonding), before studying the adhesion of brushes to epithelial cell monolayers (primary keratinocytes and HaCaT cells) and soft tissues (porcine epicardium and keratinized gingiva).

Adhesion assays to SAMs reveal the complex balance of interactions (electrostatic, van der Waals interactions and hydrogen bonding) regulating the adhesion of weak polyelectrolyte brushes. This resulted in particularly strong adhesion of PAA brushes to a wide range of surface chemistries. In turn, colloidal probe microscopy on cell monolayers highlighted the importance of the glycocalyx in regulating non-specific adhesions.

This was also reflected by the adhesive properties of soft tissues, in combination with their mechanical properties. Overall, this work clearly demonstrates the complex nature of interactions between polymeric biomaterials and biological samples and highlights the need for relatively elaborate models to predict these interactions.

TO READ THE FULL PAPER CLICK HERE

 

REQUEST A QUOTATION OR FURTHER INFORMATION

 

See what our customers have to say

  • "The technical team at Henniker are very knowledgeable and supportive and always approachable. I have found it a pleasure to work with them."

    Simon Baxter - BAE Systems, MAI

    BAE
  • "Henniker guided us to choose the most suitable plasma unit for our application, ensured an accelerated delivery time & guided us through the very easy setup. We obtained quality results with their unit within minutes of setup & consistent results thereafter. The support they have provided has been rapid and helpful."

    Dr Ravi Desai - Making Lab, Francis Crick Institute

    Francis Crick Institute
  • "Henniker provided our team with excellent service during the course of our work together, the plasma cleaner arrived quickly and was installed with ease, giving us visible results from the outset and confirming that we made the right decision in choosing a local UK manufacturer."

    Dr Panagiotis Manesiotis BSc MRSC - Queen’s University Belfast

    Queens Uni Belfast
  • "Our customers and operations demand reliability at every level and were a key factor in our decision to choose a UK based manufacturer of plasma treatment equipment."

    Tom Doak - Trak Microwave

    Queens Uni Belfast
  • "Henniker’s after sales support is first class. They have always been extremely responsive if we have ever had need to call on them."

    Steve Rackham - Teledyne

    Teledyne
  • "Henniker’s plasma systems have delivered tangible benefits to us right from day one. The team there are very easy to work with."

    Ian Bruce - Coopervision

    Coopervision
  • "Henniker really stood out, both in their product range and technical knowledge. They are a great company to work with."

    Karthik Nair - University of Bradford

    University of Bradford
  • "We are very impressed with the ease of use and reliability of our plasma unit and were producing results within minutes of setting it up."

    Dr Neil Wilson - Warwick University

    Warwick University
  • "Our collaborative work with the team at Henniker was a very positive experience and one that we look forward to developing further."

    Ewen Kellar - TWI

    TWI
  • "Henniker provided a tailored product to match our exact requirements. They are a pleasure to work with."

    Dr Will Shu - Heriot Watt University

    Heriot Watt University

  • Huf
  • Kingspan
  • BOC
  • Maclaren
  • Cambridge Uni
  • Warwick Uni
  • Morgan Ceramics
  • QMUL
  • University Bradford
  • BAE
  • Imperial College London
  • Heriot Watt Uni
Henniker Plasma Logo

ISO logo

 

 

Connect with us

Henniker Plasma 3 Berkeley Court, Manor Park, Runcorn, WA7 1TQ, UK

+44 (0)1925 830 771   +44 (0)1925 800 035     info@plasmatreatment.co.uk